Cursor myCursor = new Cursor(Cursor.Current.Handle);
             IntPtr colorCursorHandle = LoadCursorFromFile(@"D:\Documents and Settings\Administrator\桌面\图书馆管理系统V2.0\图书馆管理系统V2.0\图书馆管理系统V2.0\图书馆管理系统V2.0\bin\Debug\cur.ani");//鼠标图标路径      
             myCursor.GetType().InvokeMember("handle", BindingFlags.Public | BindingFlags.NonPublic | BindingFlags.Instance | BindingFlags.SetField, null, myCursor, new object[] { colorCursorHandle });
             this.Cursor = myCursor；


提示Cursor myCursor = new Cursor(Cursor.Current.Handle);未将对象引用设置到对象的实例。
为什么啊？

等……

应该是LoadFromFile的问题或是ani文件不正确或过大造成的 复制了楼主的代码 写了一个DEMO楼主可以参照下

using System;
using System.Collections.Generic;
using using System.Data;
using System.Drawing;
using System.Text;
using System.Windows.Forms;
using System.Reflection;
using System.Runtime.InteropServices;

namespace cursor
{
    public partial class Form1 : Form
    {
        public Form1()
        {
            InitializeComponent();
        }
        [DllImport("user32", EntryPoint = "LoadCursorFromFile")]
        public static extern IntPtr LoadCursorFromFile(string lpFileName);

        private void Form1_Load(object sender, EventArgs e)
        {
            Cursor myCursor = new Cursor(Cursor.Current.Handle);
            IntPtr colorCursorHandle = LoadCursorFromFile(@"E:\AppStarting.ani");//鼠标图标路径      
             myCursor.GetType().InvokeMember("handle", BindingFlags.Public | BindingFlags.NonPublic | BindingFlags.Instance | BindingFlags.SetField, null, myCursor, new object[] { colorCursorHandle });
             this.Cursor = myCursor;

        }
    }
}

DEMO图标文件

这个真的行！在看看！谢了……